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A Practical Handbook of Preparative HPLC

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This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.

·Provides practical, hands-on advice based on years of experience

·Will help ensure optimal design, equipment and separation results for your particular task

·Presents system layouts from laboratory to process scale

·Will help you to devise or improve record-keeping and documentation systems

Chromatography can be defined as the separation of mixtures by distribution between two or more immiscible phases. Some of these immiscible phases can be gas–liquid, gas–solid, liquid– liquid, liquid–solid, gas–liquid–solid and liquid–liquid–solid. Strictly speaking, a simple liquid–liquid extraction is in fact a chromatographic process. Similarly, distillation is a chromatographic process that involves separation of liquids by condensation of their respective vapours at different points in a column.

Most will remember the school science project of placing an ink blot in the centre of a filter paper and following this by dripping methylated spirits on to the ink. Watching in fascination as concentric circles of various pigments develop is probably the first and sometimes last experience of a chromatographic separation many will encounter. Like too many of our observations the essence of this experiment is to demonstrate that black ink is made up of several different pigments and the underlying process, in this case chromatography, is dismissed with blatant disregard.
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