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Immunochemical Protocols (Methods in Molecular Biology)

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Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies.

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Inventory Accounting : A Comprehensive Guide (Wiley Best Practices)
Inventory Accounting : A Comprehensive Guide (Wiley Best Practices)
The classical view of inventory data collection is that of employees filling out forms
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forwarded to a central data entry location, where hordes of clerks keypunch the data
into a central computer database. Although this was a reasonably accurate view of...
Joint Preservation of the Knee: A Clinical Casebook
Joint Preservation of the Knee: A Clinical Casebook
Comprised of clinical cases demonstrating strategies for both common and complex knee preservation, this concise, practical casebook will provide orthopedic surgeons with the best real-world strategies to properly manage the many kinds of knee injuries and disorders they may encounter. The opening section presents the knee joint as a...
Harmful Cyanobacteria (Aquatic Ecology Series)
Harmful Cyanobacteria (Aquatic Ecology Series)

This outstanding volume provides an up-to-date overview of the advances in our knowledge of harmful cyanobacteria. An essential reference for all scientists and environmental professionals interested in cyanobacterial ecology and water management.

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Palm & Treo Hacks
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Palm OS is one of the most popular handheld operating systems on the planet. From the newest Tungstens and Treos all the way down the family tree, Palms are everywhere. Although the Palm OS is simple to learn and use, there is more to it than meets the eye--from new features in the Palm to capabilities you can get with add-on...

The Pain Survival Guide: How to Reclaim Your Life (APA Lifetools)
The Pain Survival Guide: How to Reclaim Your Life (APA Lifetools)

If you suffer from chronic pain, this proven 10-step program brings hope and relief, showing you how gradual changes in specific behaviors can lead to great improvements in your ability to cope. Psychologists Turk and Winters' recommendations are based on solid research that shows what works and on their success with thousands of...

Itch, Clap, Pox: Venereal Disease in the Eighteenth-Century Imagination
Itch, Clap, Pox: Venereal Disease in the Eighteenth-Century Imagination
A lively interdisciplinary study of how venereal disease was represented in eighteenth-century British literature and art

In eighteenth-century Britain, venereal disease was everywhere and nowhere: while physicians and commentators believed the condition to be widespread, it remained shrouded in secrecy,
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