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Minicircle and Miniplasmid DNA Vectors: The Future of Non-viral and Viral Gene Transfer

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This first title on the topic provides complete coverage, including the molecular basis, production and possible biomedical applications. Written by the most prominent academic researchers in the field as well as by researchers at one of the world's leading companies in industrial production of minicircle DNA, this practical book is aimed at everyone who is directly or indirectly involved in the development of gene therapies.

After significant improvements in the field of non-viral vector development, we compiled the status of those “new plasmids” within this book. The tools for gene- or cell therapy and DNA vaccination are available in form of pure genetic material (DNA, RNA) or within more complex units (viral vectors, VLP, aggregates containing chemical substances or “simply” cells).

We gave an overview on initial non-viral approaches in 2001 with “Plasmids for Therapy and Vaccination” (edited by M. Schleef, E-Book ISBN 978-3-527-61284-0, Wiley-VCH) summarizing the different types of plasmid vectors to be used, their structure and functionality including regulatory aspects and those of making them. Later we focussed on the route of administration, pharmaceutical DNA and specific features (e.g. CpG motifs) of such: “DNA Pharmaceuticals” (edited by M. Schleef, ISBN 978-3-527-31187-3, Wiley-VCH).
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