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The HPLC Solvent Guide

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The HPLC Solvent Guide, 9780471411383 (0471411388), John Wiley & Sons, 2002

This practical work offers experimentalists an overview of the solvent's role in HPLC as well as detailed discussions of seven different solvent classes used in connection with HPLC methods. Each chapter on individual solvent classes includes a series of four tables which compile physical, chemical, and chromatographic properties; safety and health parameters; chemical structures; and availability, in terms of both supplier and performance specifications. The solvents are grouped into primary classes, followed by type of application and then by specific analyte class. Every separation is described in-depth including the sample matrix, list of analytes (separated and quantitated), chromatographic parameters used and abbreviated results. --This text refers to an out of print or unavailable edition of this title.

From the Inside Flap

  • Reviews the role of solvents in HPLC.
  • Detailed and expanded presentation reviewing all commonly-used solvent classes and chemical and physical properties.
  • Easy reference for solvent class, field of application, and specific analyte class.
  • Each separation is described fully; listing sample matrix, analytes separated and quantified, chromatographic parameters used, and abbreviated results.
  • Popular "how to" and "how not to" methodology.
  • Tables feature physical, chemical, and chromatographic properties of individual solvent classes; safety and health parameters; availability in terms of supplier and performance specifications; and chemical structures of solvents.
  • Focus is on practical applications and use of method optimization processes.

New to the 2nd edition:

  • Detailed discussion of method optimization techniques (triangulation, window diagrams).
  • The concept of method validation is presented, including functional definitions and general guidelines for validation protocols.
  • Detailed discussion of system suitability parameters, what they are, and how they are used.
  • Over 600 new abstracted articles reviewed, building on 1,100 articles in the 1st edition.
  • A key area of increased focus is natural products/botanicals/herbals.

About the Author

Paul C. Sadek is President of Analytical Consulting Laboratories, a manufacturing and research facility located in Kentwood, Michigan. Previously, he spent more than fifteen years in academia and industry, including many years in research and development with Burdick Jackson, the world's premier solvent manufacturer. Dr. Sadek also wrote Troubleshooting HPLC Systems: A Bench Manual.

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